Journal: Stem Cell Research & Therapy

Article Title: Morphometric prediction of mesenchymal stromal cell-like immunosuppressive capacity of human hair follicle dermal sheath cup cells: an implication for regenerative medicine in hair loss diseases

doi: 10.1186/s13287-025-04764-x

Figure Lengend Snippet: Isolation of DSC and phenotype of IFN-γ-activated DSCCs. A – D DSC isolation procedures. ( A ) A scalp tissue obtained by punch biopsy, ( B ) an intact hair follicle, ( C ) dissected and inverted DSC and DP before separation, and ( D ) morphological features of DSCCs at day 7 of primary culture. Dashed line: dissected location; bar: 500 μm. E , F DSCCs from three donors and two types of MSCs were activated with IFN-γ for 48 h and subjected to flow cytometric analysis using antibodies against anti-human leukocyte antigens and co-stimulatory molecules, HLA-ABC, HLA-DR, CD80, and CD86 ( E ), and immunosuppressive molecules, PD-L1, CD200, FASL, and HLA-G (F). G The mean fluorescence intensity of surface markers on DSCCs derived from three donors in the presence and absence of IFN-γ (** P < 0.01, *** P < 0.001, Student’s t -test). H IDO protein levels of IFN-γ-activated MSCs and DSCCs, and nonactivated MSCs and DSCCs extracts as measured by ELISA. Mean ± SD (* P < 0.05, *** P < 0.001, Student’s t -test)

Article Snippet: Bone marrow-derived MSCs (BM-MSCs) and adipose-derived MSCs (AD-MSCs) were purchased from PromoCell (Heidelberg, Germany) and cultured in the manufacturer’s recommended medium (Mesenchymal Stem Cell Growth Medium 2, PromoCell).

Techniques: Isolation, Fluorescence, Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Stem Cell Research & Therapy

Article Title: Morphometric prediction of mesenchymal stromal cell-like immunosuppressive capacity of human hair follicle dermal sheath cup cells: an implication for regenerative medicine in hair loss diseases

doi: 10.1186/s13287-025-04764-x

Figure Lengend Snippet: Effects of DSCCs and MSCs on T cell proliferation. A Representative flow cytometry histograms showing T cell proliferation after 5 days of co-culture with/without DSCCs or MSCs. Experimental conditions: PBMCs alone (red), with αCD3/28 (orange), with DSCCs + αCD3/28 (yellow), with BM-MSCs + αCD3/28 (green), and with AD-MSCs + αCD3/28 (blue). B Quantification of T cell proliferation showing the mean ± SD of the proliferation rates from three independent experiments in (A). C Quantification of T cell proliferation showing the mean ± SD of the proliferation rates after 5 days of co-culture in the presence/absence of DSCCs at the indicated ratio (PBMCs/DSCCs). DSCCs were obtained from three donors. (*** P < 0.001, Tukey HSD test)

Article Snippet: Bone marrow-derived MSCs (BM-MSCs) and adipose-derived MSCs (AD-MSCs) were purchased from PromoCell (Heidelberg, Germany) and cultured in the manufacturer’s recommended medium (Mesenchymal Stem Cell Growth Medium 2, PromoCell).

Techniques: Flow Cytometry, Co-Culture Assay

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Schematic illustrations of electrically potentiated MSCs (epMSCs) and their improved angiogenic potentials.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques:

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Electrical stimulation (ES) of hAD‐MSCs. (A) Schematic illustrations of in vitro experimental procedures and ES parameters. A constant voltage of 0.3 V with low frequency (LF, 1 Hz) and high frequency (HF, 100 Hz) was used for ES of hAD‐MSCs. (B) LIVE/DEAD staining images of the unstimulated MSCs (control) or MSCs stimulated with LF or HF. Green and red cells represent live and dead cells, respectively. Scale bars = 200 µm. (C) Cell viability of hAD‐MSCs ( n = 4). Cell viability was expressed as the percentage of live cells among the total (live and dead) cells. (D) Relative metabolic activity of unstimulated or electrically stimulated MSCs. Relative metabolic activity of each group was demonstrated as absorbance at 450 nm normalized by that of the unstimulated control ( n = 4).

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: In Vitro, Staining, Control, Activity Assay

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Expression of typical angiogenic markers in epMSCs. (A) Relative mRNA expression of VEGF‐A, HGF, bFGF, and IGF‐1 genes. Expression of each gene was normalized to that of GAPDH ( n = 4). (B) Quantification of VEGF‐A and HGF produced from MSCs and epMSCs ( n = 4). (C) Fold change in the mean pixel density of each growth factor produced by MSCs and epMSCs ( n = 4). CMs from MSCs and epMSCs were analyzed using a human growth factor antibody array to detect growth factor production in MSCs. Gene expression was normalized to GAPDH using the 2 −ΔΔCt method. An asterisk (*) denotes a statistically significant difference ( p < 0.05).

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: Expressing, Produced, Ab Array, Gene Expression

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Quantitative RNA sequencing analysis. (A) Volcano plot of differentially expressed genes (DEGs). The red and blue dots indicate the upregulated and downregulated genes in the epMSCs compared with those in the MSCs, respectively. (B) Hierarchical clustering analysis of DEGs between the MSCs and epSCs with a color scale indicating upregulation (red) and downregulation (blue). ( n = 3 per group). (C) Ten major gene ontology (GO) analysis of the genes of which expressions were significantly influenced by ES. (D) Gene expression profile associated with GO terms related to angiogenesis. Genes depicted in blue and red indicate upregulation and downregulation of angiogenesis, respectively.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: RNA Sequencing, Gene Expression

Journal: MedComm

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration

doi: 10.1002/mco2.70352

Figure Lengend Snippet: Proteomics analysis of MSCs and epMSCs. (A) Venn diagram illustrating the distribution of proteins identified the CM of MSCs and epMSCs. (B) Hierarchical clustering analysis of the differentially expressed proteins between MSC CM and epMSC CM with a color scale indicating downregulation (blue) and upregulation (red). (C) GO analysis highlighting the top 10 biological processes significantly altered by ES with. (D) Functional enrichment analysis of differentially expressed proteins with the top 10 significantly enriched GO terms related to signaling pathways.

Article Snippet: Human adipose‐derived MSCs (hAD‐MSCs) (PromoCell, Heidelberg, Germany) were maintained in MEM‐α (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic–antimycotic (100×; Gibco) in an incubator with 5% CO 2 at 37°C used at passage number 6.

Techniques: Functional Assay, Protein-Protein interactions